Citrus sinensis was tested for antibacterial activity against bacteria strains such as E.coli, pseudomonas aeruginosa, klebsiella pneumoniae, and staphylococcus aureus. The extraction was done with water and ethanol. Agar disc diffusion was used to test antibacterial activity in vitro method. On Escherichia coli, the antibacterial action of aqueous extract demonstrated a zone of inhibition. On the other organisms, it showed little or no zones of infection, although being seven millimeters in diameter. Inhibition with a diameter of 0-3mm. little inhibitory zones were also visible in the ethanol extract. On test organisms with diagram ranging from 1-3mm the inhibitory concentration that must be met (MIC). Using a two- flow serial dilution method, no zones of contamination were found in the ethanol and aqueous extract. The finding of this of this investigation indicated that the extract have limited promise for treating disorder induced by the organisms.
Zone of inhibition, disc diffusion method, test organisms, aqueous extract, ethanol extract
International Journal of Trend in Scientific Research and Development - IJTSRD having
online ISSN 2456-6470. IJTSRD is a leading Open Access, Peer-Reviewed International
Journal which provides rapid publication of your research articles and aims to promote
the theory and practice along with knowledge sharing between researchers, developers,
engineers, students, and practitioners working in and around the world in many areas
like Sciences, Technology, Innovation, Engineering, Agriculture, Management and
many more and it is recommended by all Universities, review articles and short communications
in all subjects. IJTSRD running an International Journal who are proving quality
publication of peer reviewed and refereed international journals from diverse fields
that emphasizes new research, development and their applications. IJTSRD provides
an online access to exchange your research work, technical notes & surveying results
among professionals throughout the world in e-journals. IJTSRD is a fastest growing
and dynamic professional organization. The aim of this organization is to provide
access not only to world class research resources, but through its professionals
aim to bring in a significant transformation in the real of open access journals
and online publishing.